#Basic Sciences

The little DNAs 🧬🤣😭 #science #stem #womeninstem #phd #neuroscience #cellculture #biology #scientist #biochemistry #health #medicine #universityofcambridge #foryoupage #fyp

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Always think (at least) one step ahead in your protocol when doing an experiment Always be thinking at least one step ahead. As needed: - Get pipet(s), pipet tips, and waste containers ready - Especially important for when you need to transfer liquid after spin (centrifugation) steps –don’t let your pellets resuspend!! - Set pipet(s) to needed volumes - Get tube racks - Get and label tubes - Annotate your notes https://youtube.com/shorts/kLodyNIwpDs More about experimenting smartly: https://bit.ly/experimenting_thoughtfully more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com   #scicomm #biochemistry #molecularbiology #biology #sciencelife #science #realtimechem

🎖️Follow🧫✨ Mastering Streaking Methods in Microbiology! ✨🧫 From Quadrant to Continuous, from T-Streak to Radial, every method is a skill that helps isolate the tiniest life forms with precision. 🔬 Quadrant Streak 🧫 → “Classic method to isolate pure colonies step by step.” T-Streak ✍️ → “Quick and efficient way to separate bacteria in just three moves.” Continuous Streak 🔄 → “A smooth single motion across agar for dilution.” Radial Streak 🌟 → “Like sun rays, spreading microbes from the center outwards.” Cross Streak ➕ → “Perfect for testing antibiotic-producing organisms against bacteria.” Spoon Streak 🥄 → “When you spread your sample evenly with a simple touch.” 👉 Which one do you always use in your lab? 👉 Which one do you still find tricky? #Microbiology #BiotechStudents #StreakPlate #LabSkills #PhDEntrancePrep #ThatCoolProfessor

Techniques for buffer exchange (Desalting columns, Dialysis, & Diafiltration): Pros, cons, & going-ons https://thebumblingbiochemist.com/365-days-of-science/bufferexchange/ & https://youtu.be/jWBDJcPzaEU The 3 D’s of buffer exchange! There are 3 main techniques for buffer exchange of proteins (or nucleic acids): Desalting (via gel filtration/size exclusion chromatography), Dialysis, and Diafiltration (typically using a spin concentrator) Here’s the gist: Desalting (Size Exclusion Chromatography/Gel Filtration (typically using “desalting columns”): The resin has tiny pores – proteins, DNA, RNA, etc. can’t get into any pores, so they flow right through, coming out with the buffer that was in the pores already. Meanwhile, the buffer they come in with is trapped in the pores. Dialysis: Uses a membrane pouch with movement of small things reversibly through via diffusion. Put your protein solution in a membrane pouch & put it in a big bath of liquid to exchange with. Little stuff diffuses in AND out, but your protein’s stuck. Since it relies on diffusion, the inner & outer buffers will reach equilibrium, so you need to use a large bath volume that you replace midway through. Diafiltration (typically using spin concentrators): Uses a membrane with forced one-way movement of small things through. You start by concentrating your protein, pulling the old buffer through (e.g., by centrifuging). Then you dilute it in new buffer, pull that through (concentrating again), dilute it again in new buffer (concentrating again), dilute it again . . . 2-5 times. Then you can resuspend in the final volume you want. Pros & Cons of different buffer exchange techniques: * Desalting: * Pros: * Can be done quickly, with large volumes * Lots of formats depending on needs: FPLC columns, gravity flow columns, spin columns, all of various sizes * Great for small volumes too * Cons: * Sample doesn’t get concentrated, might get diluted Finished in comments

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🧪 Unfamiliar scientific terms pop up from time to time. Let’s break down a couple of them!

Who can Just Convince us to come out of the lab when we are engaged in our Experiments 🧪 🧫 🥼🧬 #biotechnology #Microbiology #bioingenious